The Effect of Skeletal Muscle-Specific Creatine Treatment on ALS NMJ Integrity and Function.

Agnes Badu-Mensah, Xiufang Guo, Roxana Mendez, Hemant Parsaud, James J Hickman

International journal of molecular sciences 2023 Aug 31

filter terms:

Although skeletal muscle (hSKM) has been proven to be actively involved in Amyotrophic Lateral Sclerosis (ALS) neuromuscular junction (NMJ) dysfunction, it is rarely considered as a pharmacological target in preclinical drug discovery. This project investigated how improving ALS hSKM viability and function effects NMJ integrity. Phenotypic ALS NMJ human-on-a-chip models developed from patient-derived induced pluripotent stem cells (iPSCs) were used to study the effect of hSKM-specific creatine treatment on clinically relevant functional ALS NMJ parameters, such as NMJ numbers, fidelity, stability, and fatigue index. Results indicated comparatively enhanced NMJ numbers, fidelity, and stability, as well as reduced fatigue index, across all hSKM-specific creatine-treated systems. Immunocytochemical analysis of the NMJs also revealed improved post-synaptic nicotinic Acetylcholine receptor (AChR) clustering and cluster size in systems supplemented with creatine relative to the un-dosed control. This work strongly suggests hSKM as a therapeutic target in ALS drug discovery. It also demonstrates the need to consider all tissues involved in multi-systemic diseases, such as ALS, in drug discovery efforts. Finally, this work further establishes the BioMEMs NMJ platform as an effective means of performing mutation-specific drug screening, which is a step towards personalized medicine for rare diseases.

Citation

Agnes Badu-Mensah, Xiufang Guo, Roxana Mendez, Hemant Parsaud, James J Hickman. The Effect of Skeletal Muscle-Specific Creatine Treatment on ALS NMJ Integrity and Function. International journal of molecular sciences. 2023 Aug 31;24(17)