CRISPR-based engineering of RNA viruses.

CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.

Citation

Artem Nemudryi, Anna Nemudraia, Joseph E Nichols, Andrew M Scherffius, Trevor Zahl, Blake Wiedenheft. CRISPR-based engineering of RNA viruses. Science advances. 2023 Sep 15;9(37):eadj8277

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PMID: 37703376

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