Synthesis and DFT calculation of microbe-supported Pd nanocomposites with oxidase-like activity for sensitive detection of nitrite.

Nanozymes have emerged as the forefront of research in analytical sensing due to their promising applications. In this study, we utilized polyethyleneimine (PEI)-modified Pichia pastoris residue to synthesize microbial-based palladium nanocomposites (Pd/MMR) through simple in-situ reduction methods. The dispersed active sites of Pd nanoparticles with a size of 2.12 ± 0.49 nm that were supported by microbial biomass provided excellent oxidative enzyme-mimicking activity to Pd/MMR. The catalytic mechanism of Pd/MMR involved the combined action of 1O2, ·OH, and ·O2-, and possible reaction pathways and corresponding energy barriers were also revealed using DFT calculations. We also established a quantitative detection platform for nitrite using Pd/MMR. The platform could detect nitrite at concentrations of 10-300 μM with a detection limit of 0.27 μM, and was successfully applied to detect nitrite in real samples. These findings serve as a reference for the synthesis and application of metal nanocomposites using microorganisms. Copyright © 2023 Elsevier Ltd. All rights reserved.

Citation

Guozhen Wang, Ningning Feng, Shuguang Zhao, Leshan Song, Ying Zhang, Jiaxin Tong, Yuxing Liu, Xinke Kang, Tao Hu, Iram Ahmad Khan, Kuan Lu, Haiyan Wu, Jianping Xie. Synthesis and DFT calculation of microbe-supported Pd nanocomposites with oxidase-like activity for sensitive detection of nitrite. Food chemistry. 2024 Feb 15;434:137422

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PMID: 37703776

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