Bioinformatics analysis identifies the protective targets of omentin in mice with focal cerebral ischemia injury.

Omentin is known to play a protective role in ischemic stroke. However, its regulatory networks and downstream targets in the pathogenesis of IS are incompletely revealed now. In this study, the model of photochemical brain ischemia was constructed after omentin over-expression. 8 key differentially expressed genes (DEGs) were obtained and analyzed by transcriptome analysis. These DEGs were mainly related to the negative regulation of hormone secretion, cellular phosphate ion homeostasis, and other pathways. Moreover, the mRNA expression of predicted gene 3435 (Gm3435), ankyrin repeat domain 53 (Ankrd53), fibroblast growth factor 23 (Fgf23) and the Fgf23 protein expression were down-regulated after omentin over-expression in HT22 cells injured by oxygen-glucose deprivation (OGD). In conclusion, our findings identified 8 key DEGs regulated by omentin after IS. In vitro models, the Gm3435, Ankrd53, Fgf23 mRNA expression and the Fgf23 protein expression were further verified to consistent with the transcriptomics results.Copyright © 2023. Published by Elsevier Inc.

Citation

Wu Li, Jie Liu, Qi Zhang, Xiaojuan Ma, Jinwei Duan, Jiachen Wang, Ye Tian, Wenzhen Shi. Bioinformatics analysis identifies the protective targets of omentin in mice with focal cerebral ischemia injury. Prostaglandins & other lipid mediators. 2023 Sep 11:106780106780

PMID: 37704123

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