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β-thalassemia is a common genetic disorder with an estimated prevalence of 80-90 million carriers worldwide. As elevated hemoglobin A2 (HbA2) is a primary feature of carriers, hemoglobin fraction analysis is a common technique used for initial screening. However, pediatric reference intervals (RIs) are scarce. Hence, the aim was to establish pediatric RIs of hemoglobin fractions using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Samples were collected from assumed healthy children and adolescents of 1-18 years. Analyses were conducted using the Tosoh Automated Glycohemoglobin Analyzer HLC-723®G11 (Tosoh G11, HPLC) and the Capillarys 3 Octa (CE). Data were investigated for need of partitioning by both age (1-6 years vs. 6-18 years) and sex. In total, 189 and 196 subjects were included in the statistical analysis of HPLC and CE, respectively. The 95% RI of HbA2 was 2.00-2.90% by HPLC and 2.2-3.0% by CE. Partitioning of data was not clinically relevant by HPLC. However, partitioning by age was suggested by CE. RIs of hemoglobin fractions in individuals of 1-18 years using commercially available HPLC and CE equipment were reported. This is the first report of a pediatric RI of HbA2 using the Tosoh G11. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.


Kasper Munch Lauridsen, Helle Pilgaard Kristiansen, Anne Winther-Larsen. Pediatric reference intervals of the hemoglobin fractions HbA2, HbF and HbA0 using high-performance liquid chromatography and capillary electrophoresis. Clinica chimica acta; international journal of clinical chemistry. 2023 Sep 12;549:117557

PMID: 37709111

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