Verification of the efficacy of deciduae for determining the genetic type of mouse embryos.

Hiroyuki Imai, Naoki Iwamori, Tokuko Iwamori, Sumito Matsuya, Kiyoshi Kano, Ken Takeshi Kusakabe

Anatomia, histologia, embryologia 2024 Jan

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Mouse embryos in the early-implantation stage require manipulation under a microscope. While the extraction of DNA, RNA and proteins from a single sample allows for both determination of genetic type and analysis of gene expression, whole mount analysis is not possible. In this study, we explored the applicability of PCR using extraembryonic tissues, especially the decidual side tissue after isolating the embryos from implantation sites to establish a method for determining the genetic type of embryos. The implantation site was resected at each day from the date of vaginal plug confirmation, separated into embryos and deciduae. Genomic DNA were isolated separately from the embryos and the deciduae. PCR was performed using these genomic DNA, and the band patterns were compared after electrophoresis. As a result, we demonstrated that detecting embryo-derived cells in the decidua allows determination of the sex and presence of transgenes without harming the mouse embryos themselves, from 8.5 days of age. This method enables the determination of the genetic type of mouse embryos without damaging. This technique would expand the adaptations for analysis of mouse implanted embryos. © 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.

Citation

Hiroyuki Imai, Naoki Iwamori, Tokuko Iwamori, Sumito Matsuya, Kiyoshi Kano, Ken Takeshi Kusakabe. Verification of the efficacy of deciduae for determining the genetic type of mouse embryos. Anatomia, histologia, embryologia. 2024 Jan;53(1):e12976