CALB1: Anovel antiviral factor in chicken ileal mucus.

Danqing Lu, Yang Wu, Mei Fu, Yaotang Wu, Zhisheng Wang, Jian Lin, Qian Yang

International journal of biological macromolecules 2023 Dec 31

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Intestinal mucus is the first line of defense against pathogens and has several active components. Poultry have a short intestine, the mucus of which may contain antiviral components. We hence investigated the antiviral components of mucus and explored their mechanisms of action. Initially, we isolated chicken intestinal mucus proteins that significantly inhibited the replication of avian viruses. The ileum 10-30 kDa protein fraction showed the greatest inhibition of viral replication. Moreover, liquid chromatography-mass spectrometry revealed 12 high-abundance proteins in the ileum 10-30 kDa protein fraction. Among them, we investigated the antiviral activity of calcium binding protein 1 (CALB1). Furthermore, eukaryotically and prokaryotically expressed CALB1 significantly suppressed the replication of avian viruses, possibly by binding calcium ions and/or inducing autophagy. In conclusion, we isolated and identified CALB1 from chicken intestinal mucus, which suppressed replication of avian viruses by regulating cellular calcium-ion homeostasis and autophagy. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Citation

Danqing Lu, Yang Wu, Mei Fu, Yaotang Wu, Zhisheng Wang, Jian Lin, Qian Yang. CALB1: Anovel antiviral factor in chicken ileal mucus. International journal of biological macromolecules. 2023 Dec 31;253(Pt 3):127007