Iron insertion into coproporphyrin III-ferrochelatase complex: evidence for an intermediate distorted catalytic species.

Understanding the reaction mechanism of enzymes at the molecular level is generally a difficult task, since many parameters affect the turnover. Often, due to high reactivity and the formation of transient species or intermediates, detailed information on enzymatic catalysis is obtained by means of model substrates. Whenever possible, it is essential to confirm a reaction mechanism based on substrate analogues or model systems by using the physiological substrates. Here we disclose the ferrous iron incorporation mechanism, in solution and in crystallo, by the coproporphyrin III-coproporphyrin ferrochelatase complex from the firmicute, pathogen and antibiotic resistant, Listeria monocytogenes. Coproporphyrin ferrochelatase plays an important physiological role as the metalation represents the penultimate reaction step in the prokaryotic coproporphyrin-dependent heme biosynthetic pathway, yielding coproheme (ferric coproporphyrin III). By following the metal titration with resonance Raman spectroscopy and X-ray crystallography, we prove that upon metalation the saddling distortion becomes predominant both in the crystal and in solution. This is a consequence of the readjustment of hydrogen bond interactions of the propionates with the protein scaffold during the enzymatic catalysis. Once the propionates have established the interactions typical of the coproheme complex, the distortion slowly decreases, to reach the almost planar final product. This article is protected by copyright. All rights reserved. © 2023 The Protein Society.

Citation

Thomas Gabler, Andrea Dali, Federico Sebastiani, Paul Georg Furtmüller, Maurizio Becucci, Stefan Hofbauer, Giulietta Smulevich. Iron insertion into coproporphyrin III-ferrochelatase complex: evidence for an intermediate distorted catalytic species. Protein science : a publication of the Protein Society. 2023 Sep 24:e4788

PMID: 37743577

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