The role of Zfp352 in the regulation of transient expression of 2-cell specific genes in mouse embryonic stem cells.

Lusubilo Mwalilino, Mariko Yamane, Kei-Ichiro Ishiguro, Shingo Usuki, Mitsuhiro Endoh, Hitoshi Niwa

Genes to cells : devoted to molecular & cellular mechanisms 2023 Dec

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Mouse ES cell populations contain a minor sub-population that expresses genes specifically expressed in 2-cell stage embryos. This sub-population consists of 2-cell-gene labeled cells (2CLCs) generated by the transient activation of the 2-cell specific genes initiated by the master regulator, Dux. However, the mechanism regulating the transient expression remains largely unclear. Here we reported a novel function of Zfp352, one of the 2-cell specific genes, in regulating the 2CLC sub-population. Zfp352 encodes zinc-finger transcription factor belonging to the Klf family. Dux transiently activates Zfp352 after the activation of Zscan4c in a subset of the 2CLC subpopulation. Interestingly, in the reporter assay, the transcriptional activation of Zscan4c by Dux is strongly repressed by the co-expression of Zfp352. However, the knockout of Zfp352 resulted in the repression of a subset of the 2-cell-specific genes. These data suggest the dual roles of Zfp352 in regulating the transient activation of the 2-cell-specific genes. © 2023 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

Citation

Lusubilo Mwalilino, Mariko Yamane, Kei-Ichiro Ishiguro, Shingo Usuki, Mitsuhiro Endoh, Hitoshi Niwa. The role of Zfp352 in the regulation of transient expression of 2-cell specific genes in mouse embryonic stem cells. Genes to cells : devoted to molecular & cellular mechanisms. 2023 Dec;28(12):831-844