Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis.

Vanessa Bottino-Rojas, Anthony A James

Cold Spring Harbor protocols 2024 Oct 01

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Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced. © 2024 Cold Spring Harbor Laboratory Press.

Citation

Vanessa Bottino-Rojas, Anthony A James. Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis. Cold Spring Harbor protocols. 2024 Oct 01;2024(10):pdb.prot108194