Correlation Engine 2.0
Clear Search sequence regions


  • culex (1)
  • drosophila (2)
  • elements (3)
  • fly (1)
  • gene (1)
  • insects (1)
  • mosquito (7)
  • transgenesis (6)
  • transposons (4)
  • vinegar (1)
  • Sizes of these terms reflect their relevance to your search.

    Transposon-mediated transgenesis of mosquito vectors of disease pathogens followed the early success of transgenesis in the vinegar fly, Drosophila melanogaster The P transposable element used in Drosophila does not function canonically in mosquitoes, and repeatable, routine transgenesis in mosquitoes was not accomplished until new transposable elements were discovered and validated. A number of distinct transposons were subsequently identified that mediate the introduction of exogenous DNA in a stable and heritable manner in mosquito species, including members of the genera Aedes, Anopheles, and Culex The most versatile element, piggyBac, is functional in all of these mosquito genera, as well as in many other insects in diverse orders, and has been used extensively outside the class. Transposon-mediated transgenesis of recessive and dominant marker genes and reporter systems has been used to define functional fragments of gene control sequences, introduce exogenous DNA encoding products beneficial to medical interests, and act as "enhancer traps" to identify endogenous genes with specific expression characteristics. © 2024 Cold Spring Harbor Laboratory Press.

    Citation

    Vanessa Bottino-Rojas, Anthony A James. Mosquito Transposon-Mediated Transgenesis. Cold Spring Harbor protocols. 2024 Oct 01;2024(10):pdb.top107687

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 37816607

    View Full Text