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In Vitro Reconstitution of the Drosophila melanogaster CCR4-NOT Complex to Assay Deadenylation.
Methods in molecular biology (Clifton, N.J.) 2024Sizes of these terms reflect their relevance to your search.
The CCR4-NOT complex is a multi-subunit poly(A)-specific 3' exoribonuclease that catalyzes the deadenylation of mRNA. In this chapter, we describe procedures to express and purify recombinant Drosophila melanogaster CCR4-NOT. Furthermore, we provide protocols for preparing radioactively labeled RNA substrates and conducting in vitro deadenylation assays. © 2024. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
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Filip Pekovic, Elmar Wahle. In Vitro Reconstitution of the Drosophila melanogaster CCR4-NOT Complex to Assay Deadenylation. Methods in molecular biology (Clifton, N.J.). 2024;2723:19-45
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PMID: 37824062
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