Sandwich assay for β-lactoglobulin in infant food formula based on a hierarchically architectured antifouling capture probe and fluorescent recognition probe.

Tracing the presence of allergenic β-lactoglobulin (β-Lg) in infant foods is an urgent need, but the interference from the protein-rich matrix often hampered the detection accuracy. Here, we developed a sandwich assay for β-Lg in infant food formula based on a hierarchically architectured antifouling capture probe and fluorescent recognition probe. The antifouling capture probe was constructed from the polydopamine-coated magnetic particles (Fe3O4@PDA), which was modified with repeated glutamic acid-lysine (EK) antifouling peptide and aptamer towards β-Lg. The spatial arrangement of these ligands on the Fe3O4@PDA surface was carefully tailored. Furthermore, a fluorescent recognition probe based on aptamer-modified silica-doped carbon quantum dot was developed to explore a sandwich assay for β-Lg with the capture probe. The sandwich assay was proved to have high potential in detecting β-Lg in commercially available infant food samples. The work provided a new approach to developing detection methods with matrix interference-resistant properties. Copyright © 2023 Elsevier Ltd. All rights reserved.

Citation

Huan Li, Donghui Jia, Jiangbing Shuai, Xiaofeng Zhang, Shunyu Wang, Min Wang, Ke Li, Linglin Fu. Sandwich assay for β-lactoglobulin in infant food formula based on a hierarchically architectured antifouling capture probe and fluorescent recognition probe. Food chemistry. 2024 Mar 15;436:137741

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PMID: 37862989

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