Germination of aged oat seeds associated with changes in antioxidant enzyme activity and storage compounds mobilization.

Kun Yi, Jiaming Yue, Shuangfeng Yang, Yiwei Jiang, Liu Hong, Hanguo Zeng, Kai Wei, Peisheng Mao, Yan Sun, Liru Dou, Manli Li

Physiologia plantarum 2023 Sep-Oct

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Germination of aged seeds may be associated with specific metabolic changes. The objective of this study was to examine physiological and metabolic alterations before and after germination of control and aged oat (Avena sativa) seeds. The activity of antioxidant enzymes and the level of storage compounds were measured in the embryo and endosperm at 0, 4, 16, and 32 h of imbibition for control seeds and 0, 4, 16, 32, and 60 h of imbibition for medium vigor seeds after artificially accelerated aging; metabolomic changes were determined in embryos at 16 and 32 h of seed imbibition. In aged oat seeds, superoxide dismutase activity and catalase activity increased in the late imbibition stage. The content of soluble sugars decreased significantly in the later stages of imbibition, while the content of proteins increased in 32 h of seed imbibition eventually producing mannitol and proline. The mobilization of fat in deteriorated seeds was mainly through the sphingolipid metabolic pathway generated by cell growth-promoting dihydrosphingosine-1-phosphate. Ascorbic acid, avenanthramide and proline levels increased significantly at 60 h of imbibition, playing an important role in the germination of aged oat seeds. © 2023 Scandinavian Plant Physiology Society.

Citation

Kun Yi, Jiaming Yue, Shuangfeng Yang, Yiwei Jiang, Liu Hong, Hanguo Zeng, Kai Wei, Peisheng Mao, Yan Sun, Liru Dou, Manli Li. Germination of aged oat seeds associated with changes in antioxidant enzyme activity and storage compounds mobilization. Physiologia plantarum. 2023 Sep-Oct;175(5):e14020