Isolation and conformational analysis of the Gα α-helical domain.

Donghee Ham, Donghoon Ahn, Chiwoon Chung, Ka Young Chung

Biochemical and biophysical research communications 2023 Dec 10

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Heterotrimeric G proteins (G proteins), composed of Gα, Gβ, and Gγ subunits, are the major downstream signaling molecules of the G protein-coupled receptors. Upon activation, Gα undergoes conformational changes both in the Ras-like domain (RD) and the α-helical domain (AHD), leading to the dissociation of Gα from Gβγ and subsequent regulation of downstream effector proteins. Gα RD mediate the most of classical functions of Gα. However, the role of Gα AHD is relatively not well elucidated despite its much higher sequence differences between Gα subtypes than those between Gα RD. Here, we isolated AHD from Gαs, Gαi1, and Gαq to provide tools for examining Gα AHD. We investigated the conformational dynamics of the isolated Gα AHD compared to those of the GDP-bound Gα. The results showed higher local conformational dynamics of Gα AHD not only at the domain interfaces but also in regions further away from the domain interfaces. This finding is consistent with the conformation of Gα AHD in the receptor-bound nucleotide-free state. Therefore, the isolated Gα AHD could provide a platform for studying the functions of Gα AHD, such as identification of the Gα AHD-binding proteins. Copyright © 2023 Elsevier Inc. All rights reserved.

Citation

Donghee Ham, Donghoon Ahn, Chiwoon Chung, Ka Young Chung. Isolation and conformational analysis of the Gα α-helical domain. Biochemical and biophysical research communications. 2023 Dec 10;685:149153