Lan Yang, Guo-Cheng Mei, Jia-Xuan Wu, Qiao-Zhi Jiang, Zhi-Heng Su, Hai-Qing Liao, Zhen-Min Liu, Ren-Chuan Tao, Xiang-Zhi Yong
Microbes and infection 2024 Jan-FebThis study aimed to investigate the impact of Corydalis Saxicola Bunting Total Alkaloid (CSBTA) on Porphyromonas gingivalis internalization within macrophages and explore the potential role of Toll-Like Receptor 2 (TLR2) in this process. We established a P. gingivalis internalization model in macrophages by treating P. gingivalis-infected macrophages (MOI=100:1) with 200 μg/mL metronidazole and 300 μg/mL gentamicin for 1 h. Subsequently, the model was exposed to CSBTA at concentrations of 0.02 g/L or 1 μg/mL Pam3CSK4. After a 6 h treatment, cell lysis was performed with sterile water to quantify bacterial colonies. The mRNA expressions of TLR2 and interleukin-8 (IL-8) in macrophages were analyzed using RT-qPCR, while their protein levels were assessed via Western blot and ELISA respectively. P. gingivalis could internalize into macrophages and enhance the expression of TLR2 and IL-8. Activation of TLR2 by Pam3CSK4 contributed to P. gingivalis survival within macrophages and increased TLR2 and IL-8 expression. Conversely, 0.02 g/L CSBTA effectively cleared intracellular P. gingivalis, achieving a 90 % clearance rate after 6 h. Moreover, it downregulated the expression of TLR2 and IL-8 induced by P. gingivalis. However, the inhibitory effect of CSBTA on the internalized P. gingivalis model was attenuated by Pam3CSK4. CSBTA exhibited the ability to reduce the presence of live intracellular P. gingivalis and lower IL-8 expression in macrophages, possibly by modulating TLR2 activity. Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Lan Yang, Guo-Cheng Mei, Jia-Xuan Wu, Qiao-Zhi Jiang, Zhi-Heng Su, Hai-Qing Liao, Zhen-Min Liu, Ren-Chuan Tao, Xiang-Zhi Yong. Corydalis saxicolaBunting total alkaloid eliminates Porphyromonas gingivalis strain 33277 internalized into macrophages by inhibition of TLR2. Microbes and infection. 2024 Jan-Feb;26(1-2):105244
PMID: 37914020
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