Elucidating the novel mechanisms of molecular chaperones by single-molecule technologies.

Molecular chaperones play central roles in sustaining protein homeostasis and preventing protein aggregation. Most studies of these systems have been performed in bulk, providing averaged measurements, though recent single-molecule approaches have provided an in-depth understanding of the molecular mechanisms of their activities and structural rearrangements during substrate recognition. Chaperone activities have been observed to be substrate specific, with some associated with ATP-dependent structural dynamics and others via interactions with co-chaperones. This Review aims to describe the novel mechanisms of molecular chaperones as revealed by single-molecule approaches, and to provide insights into their functioning and its implications for protein homeostasis and human diseases. Copyright © 2023 Elsevier Ltd. All rights reserved.

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Ayush Chandrakant Mistry, Debojyoti Chowdhury, Soham Chakraborty, Shubhasis Haldar. Elucidating the novel mechanisms of molecular chaperones by single-molecule technologies. Trends in biochemical sciences. 2024 Jan;49(1):38-51

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PMID: 37980187

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