Evidence for paraquat-pepsin interaction: In vitro and silico study.

Najimeh Farokhvand, Behzad Shareghi, Sadegh Farhadian

Chemosphere 2024 Feb

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The use of the herbicide paraquat (PQ) has raised concerns about potential environmental consequences due to its toxicity and persistence in the environment. Considering the affinity of dangerous compounds to biological molecules, it is necessary to know their binding properties. This article focuses on the behavior of the pepsin enzyme following its contact with paraquat poison, and the interaction between paraquat and pepsin has been investigated in laboratory conditions and simulated physiological conditions using multispectral techniques. Fluorescence experiments showed that PQ uses a static method to quench pepsin's intrinsic fluorescence. By causing structural damage to pepsin, PQ may be detrimental as it alters its conformational function based on FT-IR spectroscopy. The coupling reaction is a spontaneous process caused by hydrogen bonding and van der Waals forces according to the analysis of the thermodynamic parameters of each system at three different temperatures. The molecular structure of pepsin changes when it binds to PQ. Also, the results showed that PQ is a pepsin inhibitor that changes the function of the enzyme. Copyright © 2023 Elsevier Ltd. All rights reserved.

Citation

Najimeh Farokhvand, Behzad Shareghi, Sadegh Farhadian. Evidence for paraquat-pepsin interaction: In vitro and silico study. Chemosphere. 2024 Feb;349:140714