CD271 promotes proliferation and migration in bladder cancer.

Bladder cancer is a urothelial cancer and effective therapeutic strategies for its advanced stages are limited. Here, we report that CD271, a neurotrophin receptor, promotes the proliferation and migration of bladder cancer cells. CD271 knockdown decreased proliferation in both adherent and spheroid cultures, and vice versa when CD271 was overexpressed in bladder cancer cell lines. CD271 depletion impaired tumorigenicity in vivo. Migration activity was reduced by CD271 knockdown and TAT-Pep5, a known CD271-Rho GDI-binding inhibitor. Apoptosis was induced by CD271 knockdown. Comprehensive gene expression analysis revealed alterations in E2F- and Myc-related pathways upon CD271 expression. In clinical cases, patients with high CD271 expression showed significantly shortened overall survival. In surgically resected specimens, pERK, a known player in proliferation signaling, colocalizes with CD271. These data indicate that CD271 is involved in bladder cancer malignancy by promoting cell proliferation and migration, resulting in poor prognosis. © 2023 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

Citation

Shingo Myoen, Mai Mochizuki, Rie Shibuya-Takahashi, Haruna Fujimori, Norihisa Shindo, Kazunori Yamaguchi, Jun Yasuda, Jiro Abe, Takayuki Imai, Ikuro Sato, Hisanobu Adachi, Sadafumi Kawamura, Akihiro Ito, Keiichi Tamai. CD271 promotes proliferation and migration in bladder cancer. Genes to cells : devoted to molecular & cellular mechanisms. 2024 Jan;29(1):73-85

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PMID: 38016691

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