Rapid Identification of Peptide-Receptor-Coreceptor Complexes in Protoplasts.

Xiaoyang Wang, Xiangzong Meng

Methods in molecular biology (Clifton, N.J.) 2024

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Secreted signaling peptides, also called peptide hormones, play crucial roles in regulating plant growth, development, and immunity. Plant peptide hormones are perceived by plasma membrane-localized receptor-like kinases (RLKs) or receptor-like proteins (RLPs) that harbor specific extracellular domains to bind and recognize the corresponding peptide ligands. Binding of a peptide ligand to its receptor usually induces the hetero-dimerization of the cognate receptor and a coreceptor, followed by the phosphorylation and activation of the receptor complex to transduce downstream signaling. Therefore, matching peptide ligands with their respective receptors/coreceptors is crucial for elucidating peptide hormone signaling pathways. In this chapter, using the RGF7 peptide-RGI4/RGI5 receptor-BAK1 coreceptor complex as an example, we describe a rapid method to identify the peptide ligand-receptor-coreceptor complexes via co-immunoprecipitation assays using recombinant proteins transiently expressed in Arabidopsis protoplasts. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Xiaoyang Wang, Xiangzong Meng. Rapid Identification of Peptide-Receptor-Coreceptor Complexes in Protoplasts. Methods in molecular biology (Clifton, N.J.). 2024;2731:241-251