TFP5, a Peptide Derived from Cdk5 Activator p35, Protects Pancreatic β Cells from Glucose Toxicity.

S-Y Liu, S-L Cao, H-Y Luo, L Bao, J E, B Li, X-M Lan, G-Q Zhang, X Bao, Y-L Zheng

Bulletin of experimental biology and medicine 2023 Nov

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We studied the effect of TFP5 on MIN6 cells (cultured mouse islet β cells) treated with different concentrations of glucose (5 or 25 mM). The results were verified in C57BL/6J mice (control; n=12) and db/db mice with type 2 diabetes mellitus (n=12). To synthesize TFP5, peptide p5 (a derivative of p35 protein, activator of cyclin-dependent kinase 5, Cdk5) was conjugated with a FITC tag at the N-terminus and an 11-amino acid TAT protein transduction domain at the C-terminus. TFP5 was employed to inhibit Cdk5 activity and then to evaluate its efficiency in treating experimental type 2 diabetes mellitus. TFP5 effectively inhibited the pathological hyperactivity of Cdk5, enhanced insulin secretion, and protected pancreatic β cells from apoptosis in vitro and in vivo. In addition, TFP5 inhibited inflammation in pancreatic islets by reducing the expression of inflammatory cytokines TGF-β1, TNFα, and IL-1β. These novel data indicates that TFP5 is a promising candidate for treatment of type 2 diabetes mellitus. © 2023. Springer Science+Business Media, LLC, part of Springer Nature.

Citation

S-Y Liu, S-L Cao, H-Y Luo, L Bao, J E, B Li, X-M Lan, G-Q Zhang, X Bao, Y-L Zheng. TFP5, a Peptide Derived from Cdk5 Activator p35, Protects Pancreatic β Cells from Glucose Toxicity. Bulletin of experimental biology and medicine. 2023 Nov;176(1):19-25