Clear Search sequence regions


  • acupoint (1)
  • carfilzomib (10)
  • cellular (1)
  • Col1a1 (1)
  • control group (2)
  • DEGs (8)
  • dmso (1)
  • Fxyd3 (1)
  • growth factor (1)
  • heart (3)
  • mice (3)
  • multiple myeloma (1)
  • Myl4 (1)
  • Myl7 (1)
  • oligopeptides (2)
  • PC6 (3)
  • pc6 extract (1)
  • plant extracts (2)
  • Tmem163 (1)
  • Wnt (1)
  • Sizes of these terms reflect their relevance to your search.

    Carfilzomib (CFZ), a proteasome inhibitor commonly used in the treatment of multiple myeloma (MM), exhibits limited clinical application due to its cardiotoxicity. In our study, electroacupuncture (EA) at Neiguan acupoint (PC6) effectively reversed CFZ-induced reduction in ejection fraction (EF) and fractional shortening (FS), demonstrating great potential effect for heart protection. Through comparative analysis of the transcriptome profile from heart samples of mice treated with DMSO control, CFZ injection, and EA stimulation, we identified a total of 770 differentially expressed genes (DEGs) in CFZ (vs. Control) group and 329 DEGs in EA (vs. CFZ) group. Specifically, CFZ (vs. Control) group exhibited 65 up-regulated DEGs and 705 down-regulated DEGs, while EA (vs. CFZ) group displayed 251 up-regulated DEGs and 78 down-regulated DEGs. Metascape analysis revealed that among these treatment groups, there were 137 co-expressed DEGs remarkably enriched in skeletal system development, cellular response to growth factor stimulus, negative regulation of Wnt signaling pathway, and muscle contraction. The expression patterns of miR-8114, Myl4, Col1a1, Tmem163, Myl7, Sln, and Fxyd3, which belong to the top 30 DEGs, were verified by quantitative real-time PCR (RT-qPCR). In summary, this study firstly discloses novel insights into the regulatory mechanisms underlying PC6-based EA therapy against CFZ-induced cardiotoxicity, potentially serving as a theoretical foundation for further clinical applications. Copyright © 2023 Elsevier B.V. All rights reserved.

    Citation

    Yuxuan Chen, Rou Peng, Yi Qian, Yizhou Lu, Liyao Chen, Meiling Yu, Minjiao Jiang, Wei Wu, Shengfeng Lu. Genome-wide analysis in PC6 electroacupuncture to ameliorate carfilzomib-induced cardiotoxicity in mice. Gene. 2024 Mar 01;897:148090

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 38110043

    View Full Text