Combined Effects of Pressure and Ionic Strength on Protein-Protein Interactions: An Empirical Approach.

Brian Paul, Eric M Furst, Abraham M Lenhoff, Norman J Wagner, Susana C M Teixeira

Biomacromolecules 2024 Jan 08

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Proteins are exposed to hydrostatic pressure (HP) in a variety of ecosystems as well as in processing steps such as freeze-thaw, cell disruption, sterilization, and homogenization, yet pressure effects on protein-protein interactions (PPIs) remain underexplored. With the goal of contributing toward the expanded use of HP as a fundamental control parameter in protein research, processing, and engineering, small-angle X-ray scattering was used to examine the effects of HP and ionic strength on ovalbumin, a model protein. Based on an extensive data set, we develop an empirical method for scaling PPIs to a master curve by combining HP and osmotic effects. We define an effective pressure parameter that has been shown to successfully apply to other model protein data available in the literature, with deviations evident for proteins that do not follow the apparent Hofmeister series. The limitations of the empirical scaling are discussed in the context of the hypothesized underlying mechanisms.

Citation

Brian Paul, Eric M Furst, Abraham M Lenhoff, Norman J Wagner, Susana C M Teixeira. Combined Effects of Pressure and Ionic Strength on Protein-Protein Interactions: An Empirical Approach. Biomacromolecules. 2024 Jan 08;25(1):338-348