Bioencapsulation of Oocytes and Granulosa Cells.

A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Massimo Faustini, Stella Agradi, Daniele Vigo, Maria L Torre, Giulio Curone. Bioencapsulation of Oocytes and Granulosa Cells. Methods in molecular biology (Clifton, N.J.). 2024;2749:103-108

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PMID: 38133778

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