Protocol for the detection of defined T cell clones in a heterogeneous cell population.

Identifying defined T cell clones within a polyclonal population is key to clarifying their phenotype and function. Here, we present a protocol for detecting specified T cell clones in a heterogeneous cell population. We describe steps for stimulating human CD4+ T cells isolated from blood with a protein antigen, sorting antigen-specific cells by fluorescence-activated cell sorting, and detecting among these the presence of predefined T cell clones, based on their T cell receptor (TCR). TCR cDNA is amplified through 5'-RACE (TCR-SMART) and detected by qPCR. For complete details on the use and execution of this protocol, please refer to Notarbartolo et al. (2021).1. Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Andrea Gobbini, Alessandra Bandera, Renata Grifantini, Sergio Abrignani, Samuele Notarbartolo. Protocol for the detection of defined T cell clones in a heterogeneous cell population. STAR protocols. 2024 Mar 15;5(1):102787

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PMID: 38141168

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