The OL101 O antigen locus specifies a novel Klebsiella pneumoniae serotype O13 structure.

Daria Artyszuk, Wojciech Jachymek, Radosław Izdebski, Marek Gniadkowski, Jolanta Lukasiewicz

Carbohydrate polymers 2024 Feb 15

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Klebsiella pneumoniae is one of the priority objects for the development of new therapies against infections. The species has been perceived as of limited variety of O antigens (11 O serotypes identified to date). That trait makes lipopolysaccharide an attractive target for protective antibodies. Nowadays, K. pneumoniae O antigens encoding genes are often analysed by bioinformatic tools, such as Kaptive, indicating higher actual diversity of the O antigen loci. One of the novel K. pneumoniae O loci for which the antigen structure has not been elucidated so far is OL101. In this study, four clinical isolates predicted as OL101 were characterized and found to have the O antigen structure composed of β-Kdop-[→3)-α-l-Rhap-(1→4)-α-d-Glcp-(1→]n, representing a novel serotype O13. Identification of the β-Kdop terminus was based on the analysis of the complete LPS molecule by the HR-MAS NMR spectroscopy. The bioinformatic analysis of 71,377 K. pneumoniae genomes from public databases (July 2023) revealed a notable OL101 prevalence of 6.55 %. Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.

Citation

Daria Artyszuk, Wojciech Jachymek, Radosław Izdebski, Marek Gniadkowski, Jolanta Lukasiewicz. The OL101 O antigen locus specifies a novel Klebsiella pneumoniae serotype O13 structure. Carbohydrate polymers. 2024 Feb 15;326:121581