Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

Bacteria use type III secretion injectisomes to inject effector proteins into eukaryotic target cells. Recruitment of effectors to the machinery and the resulting export hierarchy involve the sorting platform. These conserved proteins form pod structures at the cytosolic interface of the injectisome but are also mobile in the cytosol. Photoactivated localization microscopy in Yersinia enterocolitica revealed a direct interaction of the sorting platform proteins SctQ and SctL with effectors in the cytosol of live bacteria. These proteins form larger cytosolic protein complexes involving the ATPase SctN and the membrane connector SctK. The mobility and composition of these mobile pod structures are modulated in the presence of effectors and their chaperones, and upon initiation of secretion, which also increases the number of injectisomes from ~5 to ~18 per bacterium. Our quantitative data support an effector shuttling mechanism, in which sorting platform proteins bind to effectors in the cytosol and deliver the cargo to the export gate at the membrane-bound injectisome. © 2024. The Author(s).

Citation

Stephan Wimmi, Alexander Balinovic, Corentin Brianceau, Katherine Pintor, Jan Vielhauer, Bartosz Turkowyd, Carlos Helbig, Moritz Fleck, Katja Langenfeld, Jörg Kahnt, Timo Glatter, Ulrike Endesfelder, Andreas Diepold. Cytosolic sorting platform complexes shuttle type III secretion system effectors to the injectisome in Yersinia enterocolitica. Nature microbiology. 2024 Jan;9(1):185-199

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 38172622

View Full Text