Oral vaccination with recombinant Saccharomyces cerevisiae expressing Micropterus salmoides rhabdovirus G protein elicits protective immunity in largemouth bass.

Mao-Xia Yang, Jia-Hui Liang, Sheng Luo, Shi-Bo Zhang, Qian-Jin Zhou, Jian-Fei Lu, Jiong Chen

Fish & shellfish immunology 2024 Feb

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Micropterus salmoides rhabdovirus (MSRV) is one of the main pathogens of largemouth bass, leading to serious economic losses. The G protein, as the only envelope protein present on the surface of MSRV virion, contains immune-related antigenic determinants, thereby becoming the primary target for the design of MSRV vaccines. Here, we displayed the G protein on the surface of yeast cells (named EBY100/pYD1-G) and conducted a preliminary assessment of the protective efficacy of the recombinant yeast vaccine. Upon oral vaccination, a robust immune response was observed in systemic and mucosal tissue. Remarkably, following the MSRV challenge, the relative percent survival of EBY100/pYD1-G treated largemouth bass significantly increased to 66.7 %. In addition, oral administration inhibited viral replication and alleviated the pathological symptoms of MSRV-infected largemouth bass. These results suggest that EBY100/pYD1-G could be used as a potential oral vaccine against MSRV infection. Copyright © 2024 Elsevier Ltd. All rights reserved.

Citation

Mao-Xia Yang, Jia-Hui Liang, Sheng Luo, Shi-Bo Zhang, Qian-Jin Zhou, Jian-Fei Lu, Jiong Chen. Oral vaccination with recombinant Saccharomyces cerevisiae expressing Micropterus salmoides rhabdovirus G protein elicits protective immunity in largemouth bass. Fish & shellfish immunology. 2024 Feb;145:109364