Co-factor prosthesis facilitates biosynthesis of azido-pseudaminic acid probes for use as glycosyltransferase reporters.

Truncated thioester N,S-diacetylcysteamine (SNAc) was utilised as a co-factor mimic for PseH, an acetyl-coA dependent aminoglycoside N-acetyltransferase, in the biosynthesis of the bacterial sugar, pseudaminic acid. Additionally, an azido-SNAc analogue was used to smuggle N7-azide functionality into the pseudaminic acid backbone, facilitating its use as a reporter of pseudaminyltransferase activity.

Citation

Tessa Keenan, Harriet S Chidwick, Matthew Best, Emily K P Flack, Nicholas D J Yates, Natasha E Hatton, Matthew E Warnes, Martin A Fascione. Co-factor prosthesis facilitates biosynthesis of azido-pseudaminic acid probes for use as glycosyltransferase reporters. Chemical communications (Cambridge, England). 2024 Feb 01;60(11):1428-1431

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PMID: 38205715

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