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    Microbial rhodopsins are retinal membrane proteins that found a broad application in optogenetics. The oligomeric state of rhodopsins is important for their functionality and stability. Of particular interest is the oligomeric state in the cellular native membrane environment. Fluorescence microscopy provides powerful tools to determine the oligomeric state of membrane proteins directly in cells. Among these methods is quantitative photoactivated localization microscopy (qPALM) allowing the investigation of molecular organization at the level of single protein clusters. Here, we apply qPALM to investigate the oligomeric state of the first and most used optogenetic tool Channelrhodopsin-2 (ChR2) in the plasma membrane of eukaryotic cells. ChR2 appeared predominantly as a dimer in the cell membrane and did not form higher oligomers. The disulfide bonds between Cys34 and Cys36 of adjacent ChR2 monomers were not required for dimer formation and mutations disrupting these bonds resulted in only partial monomerization of ChR2. The monomeric fraction increased when the total concentration of mutant ChR2 in the membrane was low. The dissociation constant was estimated for this partially monomerized mutant ChR2 as 2.2±0.9 proteins/μm2 . Our findings are important for understanding the mechanistic basis of ChR2 activity as well as for improving existing and developing future optogenetic tools. © 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

    Citation

    Ekaterina Bestsennaia, Ivan Maslov, Taras Balandin, Alexey Alekseev, Anna Yudenko, Assalla Abu Shamseye, Dmitrii Zabelskii, Arnd Baumann, Claudia Catapano, Christos Karathanasis, Valentin Gordeliy, Mike Heilemann, Thomas Gensch, Valentin Borshchevskiy. Channelrhodopsin-2 Oligomerization in Cell Membrane Revealed by Photo-Activated Localization Microscopy. Angewandte Chemie (International ed. in English). 2024 Mar 11;63(11):e202307555

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    PMID: 38226794

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