IL-6 and TNF are Potential Inflammatory Biomarkers in Facioscapulohumeral Muscular Dystrophy.

Anna Greco, Karlien Mul, Martin H Jaeger, Jéssica C Dos Santos, Hans Koenen, Leon de Jong, Ritse Mann, Jurgen Fütterer, Mihai G Netea, Ger J M Pruijn, Baziel G M van Engelen, Leo A B Joosten

Journal of neuromuscular diseases 2024

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FSHD is a highly prevalent inherited myopathy with a still poorly understood pathology. To investigate whether proinflammatory cytokines are associated with FSHD and which specific innate immune cells are involved in its pathology. First, we measured circulating cytokines in serum samples: IL-6 (FSHD, n = 150; HC, n = 98); TNF (FSHD, n = 150; HC, n = 59); IL-1α (FSHD, n = 150; HC, n = 66); IL-1β (FSHD, n = 150; HC, n = 98); MCP-1 (FSHD, n = 14; HC, n = 14); VEGF-A (FSHD, n = 14; HC, n = 14). Second, we tested trained immunity in monocytes (FSHD, n = 15; HC, n = 15) and NK cells (FSHD, n = 11; HC, n = 11). Next, we explored the cytokine production capacity of NK cells in response to different stimuli (FSHD, n = 39; HC, n = 22). Lastly, we evaluated the cytokine production of ex vivo stimulated MRI guided inflamed (TIRM+) and paired MRI guided non inflamed (TIRM-) muscle biopsies of 21 patients and of 8 HC muscle biopsies. We included a total of 190 FSHD patients (N = 190, 48±14 years, 49% men) and of 135 HC (N = 135, 44±15 years, 47% men). We found that FSHD patients had higher concentrations of IL-6 and TNF measured (a) in the circulation, (b) after ex-vivo stimulation of NK cells, and (c) in muscle specimens. Besides, IL-6 circulating concentrations, as well as its production by NK cells and IL-6 content of FSHD muscle specimens, showed a mild correlation with disease duration, disease severity, and muscle weakness. These results show that IL-6 and TNF may contribute to FSHD pathology and suggest novel therapeutic targets. Additionally, the activation of NK cells in FSHD may be a novel pathway contributing to FSHD pathology.

Citation

Anna Greco, Karlien Mul, Martin H Jaeger, Jéssica C Dos Santos, Hans Koenen, Leon de Jong, Ritse Mann, Jurgen Fütterer, Mihai G Netea, Ger J M Pruijn, Baziel G M van Engelen, Leo A B Joosten. IL-6 and TNF are Potential Inflammatory Biomarkers in Facioscapulohumeral Muscular Dystrophy. Journal of neuromuscular diseases. 2024;11(2):327-347