Roles of Y-27632 on sheep sperm metabolism.

To investigate the effect of Y-27632 on low-temperature metabolism of sheep sperm, different concentrations of Y-27632 were added to sheep semen at 4 °C in this experiment to detect indicators such as sperm motility, plasma membrane, acrosome, antioxidant performance, mitochondrial membrane potential (MMP), and metabolomics. The results showed that the addition of 20 µM Y-27632 significantly increased sperm motility, plasma membrane integrity rate, acrosome integrity rate, antioxidant capacity, MMP level, significantly increased sperm adenosine triphosphate (ATP) and total cholesterol content, and significantly reduced sperm Ca2+ content. In metabolomics analysis, compared with the control group, the 20 µM Y-27632 group screened 20 differential metabolites, mainly involved in five metabolic pathways, with the most significant difference in Histidine metabolism (P = 0.001). The results confirmed that Y-27632 significantly improved the quality of sheep sperm preservation under low-temperature conditions. © The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.

Citation

Yu Fu, Wenzheng Shen, Haiyu Bai, Zhiyu Zhang, Zhikun Cao, Zibo Liu, Chao Yang, Shixin Sun, Lei Wang, Yinghui Ling, Zijun Zhang, Hongguo Cao. Roles of Y-27632 on sheep sperm metabolism. Journal of animal science. 2024 Jan 03;102

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PMID: 38263469

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