Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen.
Methods in molecular biology (Clifton, N.J.) 2024Autoantibodies that recognize self-antigens are believed to have a close relationship with diseases such as autoimmune diseases, cancer, and lifestyle diseases. Analysis of autoantibodies is essential for investigating pathology mechanisms, diagnosis, and therapeutics of these diseases. We developed an autoantibody profiling assay using a cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitively detect interaction between recombinant antigen protein and autoantibody and efficiently analyze autoantibody profiling in patients' sera. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Citation
Hiroyuki Takeda. Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen. Methods in molecular biology (Clifton, N.J.). 2024;2766:107-128
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PMID: 38270871
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