Fluorescence photobleaching and recovery of fluorescein sodium in carbomer film.

This study investigated fluorescence photobleaching and the recovery of fluorescein sodium (FS)-loaded carbomer films. To mitigate errors caused by the self-quenching effect, the experiments were conducted at FS concentrations of 0.1, 0.5, and 1 wt%. The results revealed a nonlinear relationship between fluorescence intensity and FS concentration (0.1-1 wt%). Moreover, the degree and rate of photobleaching increased with FS concentration. The recovery level and recovery rate exhibited contrasting relationships with FS concentration. Higher FS concentrations were associated with a longer recovery time, which can be attributed to the prolonged irradiation, resulting in a bleached region that was larger than the initially irradiated area. This journal is © The Royal Society of Chemistry.

Citation

Yung-Sheng Lin, Hao-Yan Chen, Yih-Pey Yang. Fluorescence photobleaching and recovery of fluorescein sodium in carbomer film. RSC advances. 2024 Jan 23;14(6):3841-3844

PMID: 38274174

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