Separation of phosphatidylethanolamine from its oxidation and hydrolysis products by high-performance liquid chromatography.

M Reers, P C Schmid, W L Erdahl, D R Pfeiffer

Chemistry and physics of lipids 1986 Dec 31

filter terms:

1-Palmitoyl-2-linoleoyl-phosphatidylethanolamine degrades relatively quickly when subjected to common storage and handling procedures. The degradation products consist of compounds in which double bonds in the sn-2 position acyl chain are partially oxidized and of products arising from the hydrolysis of the acyl ester bonds. Thin-layer chromatography (TLC), which is widely utilized to isolate and to ascertain the purity of phospholipids, does not readily separate the oxidation products from the parent lipid class. High-performance liquid chromatography (HPLC), however, employing a normal phase column and an isocratic, UV-transparent solvent system, can be employed to produce a rapid analytical or preparative of phosphatidylethanolamine (PE) from these degradative impurities.

Citation

M Reers, P C Schmid, W L Erdahl, D R Pfeiffer. Separation of phosphatidylethanolamine from its oxidation and hydrolysis products by high-performance liquid chromatography. Chemistry and physics of lipids. 1986 Dec 31;42(4):315-21