Exploring potooxidative degradation pathways of harmol and harmalol alkaloids in water: effects of pH, excitation sources and atmospheric conditions.

Fernando D Villarruel, M Paula Denofrio, Tobías Schmidt de León, Rosa Erra-Balsells, Ezequiel Wolcan, Fernando S García Einschlag, Franco M Cabrerizo

Physical chemistry chemical physics : PCCP 2024 Feb 14

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This work explores the photochemical degradation of cationic species of 7-hydroxy-1-methyl-2H-pyrido[3,4-b]indole or harmol (1C) and the corresponding partially hydrogenated derivative 7-hydroxy-1-methyl-3,4-dihydro-2H-pyrido[3,4-b]indole or harmalol (2C) in aqueous solution. UV-visible absorption and fluorescence emission spectroscopy coupled with multivariate data analysis (MCR-ALS and PARAFAC), HPLC and HRESI-MS techniques were used for both quantitative and qualitative analysis. The formation of hydrogen peroxide reactive oxygen species (ROS) was quantified, and the influence of pH, oxygen partial pressure and photoexcitation source on the photochemical degradation of both compounds was assessed. The potential implications on the biosynthesis of βCs and their biological role in living systems are discussed.

Citation

Fernando D Villarruel, M Paula Denofrio, Tobías Schmidt de León, Rosa Erra-Balsells, Ezequiel Wolcan, Fernando S García Einschlag, Franco M Cabrerizo. Exploring potooxidative degradation pathways of harmol and harmalol alkaloids in water: effects of pH, excitation sources and atmospheric conditions. Physical chemistry chemical physics : PCCP. 2024 Feb 14;26(7):6068-6079