Intraperitoneal Transfer of miR-29b Containing Exosome Suppresses the Development of Peritoneal Metastases from Gastric Cancer].

Yuki Kimura, Hideyuki Ohzawa, Yuki Kaneko, Hideyo Miyato, Kentaro Kurashina, Shin Saito, Hironori Yamaguchi, Yoshinori Hosoya, Naohiro Sata, Joji Kitayama

Gan to kagaku ryoho. Cancer & chemotherapy 2023 Dec

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Although miR-29b levels in peritoneal exosomes was markedly reduced in patients with peritoneal metastases(PM), their role has not been fully clarified. Bone marrow derived mesenchymal stem cells(BMSC)were transfected with miR-29b- integrating lentivirus and exosomes isolated from culture supernatants using ultracentrifugation. The effects of the exosomes on human peritoneal mesothelial cells(HPMC)were examined in vitro. The in vivo effect of murine BMSC-derived exosomes was examined with a syngeneic PM model. Culture of HPMC with TGF-β1 decreased expression of E-cadherin and calretinin with increased expression of vimentin, totally restored by adding miR-29b-rich exosomes. The exosomes inhibited proliferation and migration of HPMC, and inhibited adhesion of gastric cancer cells to HPMC. Intraperitoneal(IP)transfer of miR- 29b-rich exosomes every 3 days markedly reduced the number of PM of a murine gastric cancer cell, YTN16P, on the mesentery of C57/BL6 mice. IP administration of miR-29b-containing exosome suppresses the development of PM of gastric cancer.

Citation

Yuki Kimura, Hideyuki Ohzawa, Yuki Kaneko, Hideyo Miyato, Kentaro Kurashina, Shin Saito, Hironori Yamaguchi, Yoshinori Hosoya, Naohiro Sata, Joji Kitayama. Intraperitoneal Transfer of miR-29b Containing Exosome Suppresses the Development of Peritoneal Metastases from Gastric Cancer]. Gan to kagaku ryoho. Cancer & chemotherapy. 2023 Dec;50(13):1435-1437