Clear Search sequence regions


  • gene (4)
  • gene knockout (5)
  • hydroxyl (1)
  • ions (9)
  • phosphate (1)
  • RSN1 (6)
  • strontium (9)
  • Sizes of these terms reflect their relevance to your search.

    The irradiated Saccharomyces cerevisiae (Y-7) has good biosorption ability for strontium ions. To investigate the mechanism of strontium ion bioaccumulation in Y-7, we employed CRISPR/Cas9 gene editing technology to engineer Saccharomyces cerevisiae Y-7 and knock out the RSN1 gene, successfully constructing a RSN1 gene knockout strain (Y-7-rsn1Δ). When tested for strontium ion adsorption, the Y-7-rsn1Δ strain exhibited decreased capacity for adsorbing strontium ions and increased resistance to strontium ions. The results showed that RSN1 is involved in the transport of Sr2+, and observed significant decreases in intracellular Ca2+ of Y-7-rsn1Δ, indicating a strong correlation between bioaccumulation of Sr2+ and Ca2+. This demonstrated that the adsorption of strontium ions by Y-7 is regulated by the RSN1 gene. The knockout of the RSN1 gene resulted in the shift of the peak positions of carboxyl, amino, amide, hydroxyl, and phosphate groups on the cell surface. Copyright © 2024 Elsevier Ltd. All rights reserved.

    Citation

    Jundong Feng, Liuxin Tian, Weitai Wang, Yingqing Yang, Qian Li, Liang Liu, Hongyu Bo, Chengyu He. Effect of RSN1 gene knockout on the adsorption of strontium ions by irradiated Saccharomyces cerevisiae. Journal of environmental radioactivity. 2024 Mar;273:107396

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 38325251

    View Full Text