Protocol for gene knockdown using siRNA in primary cultured neonatal murine microglia.

In this protocol, we describe the small interfering RNA (siRNA)-mediated gene knockdown in primary mouse microglia, providing an approach to investigate functions such as phagocytosis and chemotaxis. The approach includes siRNA design, establishment of mixed glial cultures, microglia isolation, and siRNA transfection. Validation of knockdown efficacy employs quantitative immunoblot analysis. This technique empowers the investigation of specific molecular and cellular functions within the intricate microenvironment of the brain, comprising diverse cell types. For complete details on the use and execution of this protocol, please refer to Iguchi et al. (2023).1. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Yuma Kato, Sho Takatori, Aika Akahori, Hayato Etani, Yung Ning Chu, Taisuke Tomita. Protocol for gene knockdown using siRNA in primary cultured neonatal murine microglia. STAR protocols. 2024 Mar 15;5(1):102867

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PMID: 38341850

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