A gene-encoded aldehyde tag repurposed from RiPP cyclophane-forming pathway.

Gene-encoded aldehyde tag technology has been widely utilized in protein bioorthogonal chemistry and biotechnological application. Herein, we report utilization of the promiscuous rSAM cyclophane synthase SjiB involved in triceptide biosynthesis as a dedicated and highly efficient formylglycine synthase. The new aldehyde tag sequence in this system, YQSSI, is biosynthetically orthogonal to the known aldehyde tag (C/S)x(P/A)xR. The potential use of SjiB/YQSSI aldehyde tag system was further validated in fluorescent labelling of model proteins. Copyright © 2024 Elsevier Ltd. All rights reserved.

Citation

Suze Ma, Heng Chen, Shuxun Liu, Xuedong Huang, Tianlu Mo, Wan-Qiu Liu, Wei Zhang, Wei Ding, Qi Zhang. A gene-encoded aldehyde tag repurposed from RiPP cyclophane-forming pathway. Bioorganic & medicinal chemistry letters. 2024 Mar 15;101:129653

Mesh Tags

Substances

PMID: 38360420

search → result