Mechanistic insight into glioma through spatially multidimensional proteomics.

Characterizing the tumor microenvironment at the molecular level is essential for understanding the mechanisms of tumorigenesis and evolution. However, the specificity of the blood proteome in localized region of the tumor and its linkages with other systems is difficult to investigate. Here, we propose a spatially multidimensional comparative proteomics strategy using glioma as an example. The blood proteome signature of tumor microenvironment was specifically identified by in situ collection of arterial and venous blood from the glioma region of the brain for comparison with peripheral blood. Also, by integrating with different dimensions of tissue and peripheral blood proteomics, the information on the genesis, migration, and exchange of glioma-associated proteins was revealed, which provided a powerful method for tumor mechanism research and biomarker discovery. The study recruited multidimensional clinical cohorts, allowing the proteomic results to corroborate each other, reliably revealing biological processes specific to gliomas, and identifying highly accurate biomarkers.

Citation

Lei Shen, Zhourui Zhang, Pengfei Wu, Jingyi Yang, Yuankun Cai, Keyu Chen, Songshan Chai, Jingwei Zhao, Hongyu Chen, Xuan Dai, Bangkun Yang, Wei Wei, Lixin Dong, Jincao Chen, Pucha Jiang, Changjun Cao, Chao Ma, Chengshi Xu, Yichun Zou, Jibo Zhang, Wenping Xiong, Zhengwei Li, Shuangxiang Xu, Bing Shu, Mengyang Wang, Zejin Li, Qiongqiong Wan, Nanxiang Xiong, Suming Chen. Mechanistic insight into glioma through spatially multidimensional proteomics. Science advances. 2024 Feb 16;10(7):eadk1721

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PMID: 38363834

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