Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression.

Marisa Connell, Yonggang Xie, Xiaobing Deng, Rui Chen, Sijun Zhu

Cell reports 2024 Mar 26

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During asymmetric division of Drosophila larval neuroblasts, the fate determinant Prospero (Pros) and its adaptor Miranda (Mira) are segregated to the basal cortex through atypical protein kinase C (aPKC) phosphorylation of Mira and displacement from the apical cortex, but Mira localization after aPKC phosphorylation is not well understood. We identify Kin17, a DNA replication and repair protein, as a regulator of Mira localization during asymmetric cell division. Loss of Kin17 leads to aberrant localization of Mira and Pros to the centrosome, cytoplasm, and nucleus. We provide evidence to show that the mislocalization of Mira and Pros is likely due to reduced expression of Falafel (Flfl), a component of protein phosphatase 4 (PP4), and defects in dephosphorylation of serine-96 of Mira. Our work reveals that Mira is likely dephosphorylated by PP4 at the centrosome to ensure proper basal localization of Mira after aPKC phosphorylation and that Kin17 regulates PP4 activity by regulating Flfl expression. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Marisa Connell, Yonggang Xie, Xiaobing Deng, Rui Chen, Sijun Zhu. Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression. Cell reports. 2024 Mar 26;43(3):113823