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The defatted seeds of evening primrose (DE), a by-product of evening primrose oil extraction, are currently underutilized. This study aimed to valorize DE by examining its effects on melanogenesis and tyrosinase activity in zebrafish embryos and in vitro, and an innovative affinity-labeled molecular networking workflow was proposed for the rapid identification of tyrosinase inhibitors in DE. Our results indicated DE significantly reduced melanin content (53.3 % at 100 μg/mL) and tyrosinse activity (80.05 % for monophenolase and 70.40 % for diphenolase at 100 μg/mL). Furthermore, through the affinity-labeled molecular networking approach, 20 compounds were identified as potential tyrosinase inhibitors within DE, predominantly flavonoids and tannins characterized by catechin and galloyl substructures. Seven of these compounds were isolated and their inhibitory effects on tyrosinase were validated using functional assays. This study not only underscores the potential of DE as a rich source of natural tyrosinase inhibitors but also establishes the effectiveness of affinity-labeled molecular networking in pinpointing bioactive compounds in complex biological matrices. Copyright © 2024 Elsevier Ltd. All rights reserved.

Citation

Zhiqiang Wang, Mingfan Cui, Hao Wang, Lei Ma, Yehong Han, Dandan Han, Hongyuan Yan. Identification of tyrosinase inhibitors in defatted seeds of evening primrose (Oenothera biennis L.) by affinity-labeled molecular networking. Food research international (Ottawa, Ont.). 2024 Mar;180:114097

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PMID: 38395549

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