Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile.

The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9 showed 99.8-100% identity with Bartonella koehlerae. Haplotype analysis yielded 29 different rpoB-B. henselae haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 rpoB-B. koehlerae haplotypes, with hap#2 represented in all 3 B. koehlerae infected cats. More than one rpoB -B. henselae and B. koehlerae haplotypes were identified in individual cats, reporting by first time coinfection by different B. henselae/B. koehlerae rpoB variants in cats from Chile. Copyright © 2024 Elsevier Ltd. All rights reserved.

Citation

Paulina Sepúlveda-García, Ronald Jara, Armin Mella, Gustavo Monti, Nivia Canales, Maria Eduarda Chiaradia Furquim, Marcos Rogério André, Ananda Müller. Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile. Comparative immunology, microbiology and infectious diseases. 2024 Apr;107:102150

Mesh Tags

PMID: 38401221

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