Preparation of ER Microsomes from Arabidopsis thaliana.

Verena Kriechbaumer

Methods in molecular biology (Clifton, N.J.) 2024

filter terms:

Microsomes are vesicles derived from the endoplasmic reticulum (ER) when cells are broken down in the lab. These microsomes are a valuable tool to study a variety of ER functions such as protein and lipid synthesis in vitro.Here we describe a protocol to isolate ER-derived microsomes Arabidopsis thaliana seedlings and exemplify the use of these purified microsomes in enzyme assays with the auxin precursors tryptophan (Trp) or indole-3-pyruvic acid (IPyA) to quantify auxin synthetic capacity in microsomal and cytosolic fractions. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.