Protocol for ovariectomy and estradiol replacement in mice.

Ovariectomy, involving the surgical removal of ovaries, and estradiol replacement facilitate the understanding of sexual dimorphism-related physiological changes, encompassing reproductive biology, metabolism, and hormone-related diseases. In this study, we present a protocol for conducting ovariectomy and estradiol replacement in mice. We describe steps for performing sham and ovariectomy operations, outline preoperative preparations, and provide details on postoperative care, including analgesia administration and the removal of surgical clips. Additionally, we elaborate on the procedures for performing vehicle and estradiol injections. For complete details on the use and execution of this protocol, please refer to Luengo-Mateos et al.1. Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

María Luengo-Mateos, Antía González-Vila, Ana María Torres Caldas, Ali M Alasaoufi, Marco González-Domínguez, Miguel López, Ismael González-García, Olga Barca-Mayo. Protocol for ovariectomy and estradiol replacement in mice. STAR protocols. 2024 Mar 15;5(1):102910

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PMID: 38416648

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