Evolution-Based Discovery of Polyketide Acylated Valine from a Cytochalasin-Like Gene Cluster in Simplicillium lamelliciola HDN13430.

Zuodong Wu, Wenxue Wang, Jilong Li, Chuanteng Ma, Liangzhen Chen, Qian Che, Guojian Zhang, Tianjiao Zhu, Dehai Li

Journal of natural products 2024 Apr 26

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Utilizing a gene evolution-oriented approach for gene cluster mining, a cryptic cytochalasin-like gene cluster (sla) in Antarctic-derived Simplicillium lamelliciola HDN13430 was identified. Compared with the canonical cytochalasin biosynthetic gene clusters (BGCs), the sla gene cluster lacks the key α,β-hydrolase gene. Heterologous expression of the sla gene cluster led to the discovery of a new compound, slamysin (1), characterized by an N-acylated amino acid structure and demonstrating weak anti-Bacillus cereus activity. These findings underscore the potential of genetic evolution in uncovering novel compounds and indicating specific adaptive evolution within specialized habitats.

Citation

Zuodong Wu, Wenxue Wang, Jilong Li, Chuanteng Ma, Liangzhen Chen, Qian Che, Guojian Zhang, Tianjiao Zhu, Dehai Li. Evolution-Based Discovery of Polyketide Acylated Valine from a Cytochalasin-Like Gene Cluster in Simplicillium lamelliciola HDN13430. Journal of natural products. 2024 Apr 26;87(4):1222-1229