The open channel state in anion channelrhodopsin GtACR1 is a red-absorbing intermediate.

Anion channelrhodopsin GtACR1 is a powerful optogenetic tool to inhibit nerve activity. Its kinetic mechanism was interpreted in terms of the bacteriorhodopsin photocycle, and the L intermediate was assigned to the open channel state. Here, we report the results of the comparison between the time dependence of the channel currents and the time evolutions of the K-like and L-like spectral forms. Based on the results, we question the current view on GtACR1 kinetics and the assignment of the L intermediate to the open channel state. We report evidence for a red-absorbing intermediate being responsible for channel opening. Copyright © 2024 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Citation

Istvan Szundi, David S Kliger. The open channel state in anion channelrhodopsin GtACR1 is a red-absorbing intermediate. Biophysical journal. 2024 Apr 16;123(8):940-946

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PMID: 38462839

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