Evaluation of Kappa-carrageenan supplementation in extender for post-thaw Kajli ram sperm quality.

Saima Qadeer, Asma Ashraf, Muhammad Umer Farooq, Sana Ullah, Muhammad Asad, Ahmad Muneeb, Muhammad Adil

Reproduction in domestic animals = Zuchthygiene 2024 Mar

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Cryopreservation is one of the reliable techniques for long-term storage of sperm. The success of this technique depends on the choice of cryoprotectant; therefore, a plethora of literature has reported the effects of different cryoprotective agents so far. Kappa-carrageenan (κ-carrageenan) is a hydrocolloid polysaccharide extracted from red marine seaweed. Its unique property makes it a promising option as a non-colligative cryoprotectant. The current study aims to evaluate the cryoprotective effect of k-carrageenan along with glycerol on ram sperm quality both after equilibration and freezing. Nine Kajli rams were utilized in this experiment for semen collection through an artificial vagina maintained at 42°C. Qualified samples were diluted in tris egg yolk glycerol (TEYG) extender containing different concentrations of k-carrageenan as 0 mg/mL (control), 0.2, 0.5, 0.8 and 1 mg/mL. Post-thaw assessment was done at 37°C after 24 h of storage, which showed a significant improvement (p < .05) in sperm viability, motility, membrane and acrosome integrity in an extender containing k-carrageenan at a concentration of 0.5 mg/mL compared to control. It is concluded from the current study that the combination of glycerol and 0.5 mg/mL concentration of k-carrageenan improved the sperm post-thaw quality. © 2024 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.

Citation

Saima Qadeer, Asma Ashraf, Muhammad Umer Farooq, Sana Ullah, Muhammad Asad, Ahmad Muneeb, Muhammad Adil. Evaluation of Kappa-carrageenan supplementation in extender for post-thaw Kajli ram sperm quality. Reproduction in domestic animals = Zuchthygiene. 2024 Mar;59(3):e14551