ALDH Activity Assay: A Method for Cancer Stem Cell (CSC) Identification and Isolation.

Vitale Del Vecchio, Marcella La Noce, Virginia Tirino

Methods in molecular biology (Clifton, N.J.) 2024

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Cancer stem cells (CSCs) are a small tumor cell subpopulation, driving cancer initiation, progression, multidrug resistance, and metastasis. Several methods are used to detect and isolate CSCs by flow cytometry. Among these, measurement of aldehyde dehydrogenase (ALDH) activity within the cell is an assay widely used to identify and isolate CSCs from different types of solid tumors. The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, overexpressed both in normal and cancer stem cells. In this chapter, it is described how CSCs are detected and isolated by using ALDH activity assay. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Vitale Del Vecchio, Marcella La Noce, Virginia Tirino. ALDH Activity Assay: A Method for Cancer Stem Cell (CSC) Identification and Isolation. Methods in molecular biology (Clifton, N.J.). 2024;2777:83-89